Jan Friedrich Busche

Optofluidic Microsystem for Antibiotic Susceptibility Testing


Front	Back

ISBN:

978-3-8440-8311-8

Series:

Schriftenreihe Mikrotechnik
Herausgeber: Prof. Dr. rer.nat. Andreas Dietzel
Braunschweig

Keywords:

Lab-on-a-chip; AST; microAST; Antimicrobial Susceptibility Testing

Type of publication:

Thesis

Language:

English

Pages:

182 pages

Figures:

57 figures

Weight:

269 g

Format:

21 x 14,8 cm

Bindung:

Paperback

Price:

48,80 € / 61,10 SFr

Published:

November 2021

Buy:

DOI:

10.2370/9783844083118 (Online document)

Download:

Available PDF-Files for this title:

You need the Adobe Reader, to open the files. Here you get help and information, for the download.

These files are not printable.


	Document		Document
	Type		PDF
	Costs		12,20 EUR
	Action		Purchase in obligation and display of file - 17,1 MB (17904280 Byte)
	Action		Purchase in obligation and download of file - 17,1 MB (17904280 Byte)


	Document		Table of contents
	Type		PDF
	Costs		free
	Action		Display of file - 749 kB (766514 Byte)
	Action		Download of file - 749 kB (766514 Byte)

User settings for registered users

You can change your address here or download your paid documents again.

User:	Not logged in.
Actions:	Login / Register Forgotten your password?

Recommendation:

You want to recommend this title?

Review copy

Here you can order a review copy.

Link

You want to link this page? Click here.

Abstract

Nosocomial infections with antimicrobial resistant bacteria are on the rise and even reserve antibiotics are losing their effectiveness. In case of a bacterial infection with the risk of sepsis, the most appropriate antibiotics must be prescribed quickly. Ineffective antibiotics risks patients’ lives, unnecessarily administered antibiotics endanger their long-term effectiveness. This would make many operations and therapies routinely performed today impossible. Currently, detection of antimicrobial resistance is often done off-site and takes too long. Rapid point-of-care testing is needed to achieve medication with effective antibiotics in time. This could be achieved with phenotypic microfluidic diagnostic methods that work label-free.
In this thesis, different approaches for bacterial immobilization and subsequent growth detection are investigated. A microfluidic immobilization principle is combined with an asymmetric diffraction grating to concentrate the bacteria and study their growth label-free under the influence of antibiotics.
Results of CFD simulations of the cross flow immobilization suggested the shear stress in the systems to be approximately half the value reported to be growth limiting for E. coli cells. In line with that, the interferometric measuring principle enabled bacterial growth detection in less than 4 h with a doubling time of 79 min and sample volumes of 187.2 μL and also allowed observing the effect of applied antibiotics label-free. To complete this concept, an improvement of the fabrication of microfluidic systems with two-photon polymerization and subsequent multiple replication is investigated for their wide-spread use.

Author profile

Jan Friedrich Busche was born in Gehrden in 1989. He successfully completed his master's degree in aerospace engineering in Braunschweig in 2015. From 2015, he was employed as a research assistant at the Institute of Microtechnology and worked primarily on the development of microfluidic systems for the detection of antibiotic resistance.