Hagen Müller

Development of an antimicrobial peptide production process platform in Pichia pastoris


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ISBN:

978-3-8440-6463-6

Series:

Schriftenreihe des Institutes für Bioverfahrenstechnik und Pharmazeutische Technologie
Herausgeber: Prof. Dr.-Ing Peter Czermak
Gießen

Volume:

Keywords:

Pichia pastoris; Fermentation; Antimicrobial peptides; Golden Gate; Elastin like Polypeptides

Type of publication:

Thesis

Language:

English

Pages:

100 pages

Figures:

37 figures

Weight:

147 g

Format:

21 x 14,8 cm

Bindung:

Paperback

Price:

39,80 € / 49,80 SFr

Published:

February 2019

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Abstract

Antimicrobial peptides (AMPs) are promising alternatives to fight antibiotic resistant microorganisms. Researchers have found more than 5000 AMPs. Efficient production processes however are scarce. One of the most promising approaches to overcome this hurdle is the recombinant protein production. The process development is quite complex and time consuming and there is a lack of strategies and workflows and thus researchers and process engineers are seeking for a systematic approach to shorten development times and decrease development costs. Therefore, the systematic approach developed in this work to build up a process development platform for AMPs utilizing the expression host Pichia pastoris (P. pastoris) innovating and highly promising.